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KMID : 0616120110420030253
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Korean Journal of Pharmacognosy
2011 Volume.42 No. 3 p.253 ~ p.259
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Anti-inflammatory Effect of Inonotus obliquus Extracts in Lipopolysaccharide-induced Mouse Peritoneal Macrophage
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Ko Suk-Kyung
Pyo Myoung-Yun
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Abstract
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Macrophages play a vital role in the innate immune system involving defensive cytokines such as TNF (tumor necrosis factor)- and nitric oxide (NO). Therefore, we try to elucidate the anti-inflammatory activity of Chaga mushroom (Inonotus Obliquus, IO) in murine macrophages. Raw 264.7 cells and peritoneal macrophages of mice were cultured with or without LPS/LPS + IFN- in the presence of IO aqueous extracts (IOE 0.2, 2, 20, 100 /mL) for 24 hr and 48 hr, respectively. Exposure of IOE caused the decrease of NO production and increase of TNF- production in dose-dependent manner in activated peritoneal macrophage in vitro. To further investigate anti-inflammatory effects of IO ex vivo, we orally administrated capsaicin (PC, 3 mg/kg/day) and IOE (100, 200, 400 mg/kg/day) for 4 consecutive days to C57BL/6 mice (7~9 weeks old, female), then observed the NO secretion and cytokine (TNF-) production of LPS/LPS + INF--stimulated peritoneal macrophages. IOE inhibits NO secretion in dose-dependent manner both ex vivo and in vitro and increases the production of TNF- in vitro. In addition, we found that IOE possessed suppressive effects of LPS-stimulated TNF-, IL-, COX-2, as well as iNOS expressions in Raw 264.7 cells. These findings indicate that IOE suppress not only the LPS-induced NO overproduction of murine peritoneal macrophages, but also iNOS, COX-2, TNF-, and IL- overexpression of LPS-induced Raw 264.7 cells. Consequently, our results suggest that IO may have the anti-inflammatory effects via suppression of the inflammatory cytokines and mediators, and be useful for the treatment of inflammatory diseases
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KEYWORD
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Inonotus obliquus, Anti-inflammatory, Macrophages, NO, TNF-, iNOS, COX-2, IL-
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